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prc cmv rev1b  (Addgene inc)


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    Structured Review

    Addgene inc prc cmv rev1b
    Prc Cmv Rev1b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prc+cmv+rev1b/pcDNA3%2E1%2FPuro-CAG-ASAP1+(Plasmid+%2352519)/pm41053501-251-36-57
    Average 93 stars, based on 29 article reviews
    prc cmv rev1b - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Chromatin protein complexes involved in gene repression in lamina-associated domains
    Article Snippet: pRC-CMV-Rev1B , Addgene , 164443.


    Transfection:

    Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist
    Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described . .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 10 5 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1 WT (Addgene plasmid #170447). ..

    Article Title: Linear ubiquitination at damaged lysosomes induces local NFKB activation and controls cell survival.
    Article Snippet: .. To stably generate MYC-APEX2-LC3B-expressing cells, HEK293T cells were transfected with pHAGE-MYC-APEX2LC3B [46] and the packaging plasmids pHDM-VSV-G, pHDM-Tat1b, pHDM-Hgpm2 and pRC-CMV-Rev1b using Lipofectamine 2000 (Thermo Fischer Scientific 13,778,030). pHDM-VSV-G (Addgene 164,440; RRID:Addgene_164440), pHDM-Tat1b (Addgene 164,442; RRID:Addgene_164442), pHDM-Hgpm2 (Addgene 164,441; RRID:Addgene_164441) and pRC-CMV-Rev1b (Addgene 164,443, RRID: Addgene_164443) were gifts from Alejandro Balazs. ..

    Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist.
    Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described67. .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 105 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1WT (Addgene plasmid #170447). ..

    Plasmid Preparation:

    Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist
    Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described . .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 10 5 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1 WT (Addgene plasmid #170447). ..

    Article Title: Viral and immunologic evaluation of smokers with severe COVID-19
    Article Snippet: .. The plasmids were obtained from Addgene under the reference names pHAGE-CMV-luc2-IRES-ZsG-W (Addgene plasmid # 164,432), pRC-CMV-Rev1b (Addgene plasmid # 164,443), pHDM-Tat1b (Addgene plasmid # 164,442), pHDM-Hgpm2 (Addgene plasmid # 164,441), pTwist-SARS-CoV-2 ∆18 (Addgene plasmid # 164,436). .. Here, 12–15 million HEK293 T cells were seeded in T175 (ThermoFisher) in presence of 25 ml of DMEM10.

    Article Title: Viral and immunologic evaluation of smokers with severe COVID-19.
    Article Snippet: .. The plasmids were obtained from Addgene under the reference names pHAGE-CMV-luc2-IRES-ZsG-W (Addgene plasmid # 164,432), pRC-CMV-Rev1b (Addgene plasmid # 164,443), pHDM-Tat1b (Addgene plasmid # 164,442), pHDM-Hgpm2 (Addgene plasmid # 164,441), pTwist-SARS-CoV-2 ∆18 (Addgene plasmid # 164,436). .. Here, 12–15 million HEK293 T cells were seeded in T175 (ThermoFisher) in presence of 25 ml of DMEM10.

    Article Title: Cross-Neutralization of Distant Coronaviruses Strongly Correlates with Spike S2-Specific Antibodies from Immunocompetent and Immunocompromised Vaccinated SARS-CoV-2-Infected Patients
    Article Snippet: The plasmids obtained from Addgene (Watertown, MA, USA) were gifted by Dr. Alejandro Balazs. .. A group of 4 plasmids—pHAGE-CMV-luc2-IRES-ZsG-W (Addgene plasmid # 164432), pRC-CMV-Rev1b (Addgene plasmid # 164443), pHDM-Tat1b (Addgene plasmid # 164442), and pHDM-Hgpm2 (Addgene plasmid # 164441)—were used for production of all pseudovirus variants. ..

    Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist.
    Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described67. .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 105 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1WT (Addgene plasmid #170447). ..

    Stable Transfection:

    Article Title: Linear ubiquitination at damaged lysosomes induces local NFKB activation and controls cell survival.
    Article Snippet: .. To stably generate MYC-APEX2-LC3B-expressing cells, HEK293T cells were transfected with pHAGE-MYC-APEX2LC3B [46] and the packaging plasmids pHDM-VSV-G, pHDM-Tat1b, pHDM-Hgpm2 and pRC-CMV-Rev1b using Lipofectamine 2000 (Thermo Fischer Scientific 13,778,030). pHDM-VSV-G (Addgene 164,440; RRID:Addgene_164440), pHDM-Tat1b (Addgene 164,442; RRID:Addgene_164442), pHDM-Hgpm2 (Addgene 164,441; RRID:Addgene_164441) and pRC-CMV-Rev1b (Addgene 164,443, RRID: Addgene_164443) were gifts from Alejandro Balazs. ..



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